METHOD OF ROTATABLE CENTRAL COMPOSITION PLANNING OF THE EXPERIMENT IN THE STUDY OF EFFICIENCY DISINFECTANTS

This article discusses the effect of the concentration of active components on the effectiveness of disinfectants with using of the rotatable central compositional design of the experiment. Efficacy studied on E. coli culture. There was determinate optimal percentage of the chemical composition for obtaining disinfectants based on aldehydes, amines, chlorine-containing substances: Didecylammonium chloride -9.8%; Glutaraldehyde -9.7%; glyoxal 7.7%; distilled water - 66.8%; auxiliary components - 6%. It was found that the greatest influence on the effectiveness of the disinfectant is exerted by the pairwise interactions of the components and not the content of the components separately. The exception is distilled water. Under these conditions, the death of E. coli is 98.1 ± 1.6% (found in the experiment). The theoretically calculated value (according to the equation) was 95.4%. The deviation of the experimentally found value from the theoretical was 2.75%, which indicates the adequacy of the model.
The study of a new designed disinfectant allowed us to conclude that it is effective for: St. аureus ATCC 6538 starting at concentration of 0, 25% with an exposure of 60 minutes; Y. pestis EV и V. cholerae 0I 75- М at concentration of at least 2% of the exposure for 60 minutes; C. аlbicans АТСС 10231 at a concentration of 0.5% when exposed to 15 minutes; B. anthracis Tsenkovsky 4% solution for 60 minutes. DS according to the degree of exposure to the body, toxicity belongs to the 3rd class of moderately hazardous substances when introduced into the stomach and the 4th class of low-hazard substances when applied to the skin.

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