Determination of biomarkers of buckwheat honey By solid-phase microextraction in order to establish authenticity

Honey production in our country has increased compared to the last decade. East Kazakhstan, Almaty and South Kazakhstan regions are the main producers of honey. The floral diversity of our country makes it possible to obtain many types of monofloral honey and several combinations of polyfloral honey. Floral origin is one of the most important factors influencing the honey market. Monofloral honey is more popular among consumers due to its taste, special characteristics and is more profitable for producers due to its high price. Darker honey (buckwheat honey) usually contains high amounts of antioxidants, which increase its health potential. Food control is a major factor for consumers who want to know that their purchases are of high quality. To ensure that the actual composition of the products indicated on the label, analysis and development of effective methods of analysis is necessary. Therefore, the aim of this study is to identify biomarkers of buckwheat honey by vacuum solid phase microextraction (vac-SPME) in combination with gas chromatography-mass spectrometry (GC-MS).

1. Missio da Silva P., Gauche C., Valdemiro Gonzaga L., Costa C.O.A., Fett R. Honey: chemical composition, stability and authenticity, Food Chemistry 196, 2016.

2. Plutowska B., Chmiel T., Dymerski T., Wardencki W. A Headspace Solid-Phase Microextraction Method Development and Its Application in the Determination of Volatiles in Honeys by Gas Chromatography, Food Chemistry, 126(3): 1288–98, 2011.

3. FAOSTAT. Food and agriculture organization of the United Nations, commodity balances-livestock and fish primary equivalent, 2018 http://www.fao.org/faostat.

4. Honey, Pollinators and Bees, Food and agriculture organization of the United Nation infographics collection, 2019. http://www.fao.org/resources/infographics/infographics-details/en/c/1202954/.

5. Li L., Lietz G., Seal C. Buckwheat and CVD Risk Markers: A Systematic Review and Meta-Analysis. Nutrients, 10(5):619, 2018.

6. Dżugan M., Tomczyk M., Sowa P., Grabek-Lejko D. Antioxidant Activity as Biomarker of Honey Variety. Molecules, 2018;23(8):2069, Published 2018 Aug 18. doi:10.3390/molecules23082069.

7. Arroyo-Manzanares N., Garcia-Nicolas M., Castell A., Campillo N., Vinas P., Lopez-Garcia I., Hernandez-Cordoba M. Untargeted headspace gas chromatography – Ion mobility spectrometry analysis for detection of adulterated honey; Talanta 205, 2019, pp. 120–123. https://doi.org/10.1016/j.talanta.2019.120123

8. Pasini F., Gardini S., Marcazzan G.L., Caboni M.F. Buckwheat Honeys: Screening of Composition and Properties. Food Chemistry, 2013, 141 (3): 2802–11. https://doi.org/https://doi.org/10.1016/j.foodchem.2013.05.102.

9. Wolski T., Krzysztof T., Rybak-Chmielewska H., Kedzia B. Identification of honey volatile components by solid phase microextraction (SPME) and gas chromatography /mass spectrometry (GC/MS), 2006.

10. Psillakis E. Vacuum-assisted headspace solid-phase microextraction: A tutorial review. Analytica Chimica Acta, 986, 2017, pp. 12–24.

11. Psillakis E., Yiantzi E., Sanchez-Prado L., Kalogerakis N. Vacuum-assisted headspace solid phase microextraction: Improved extraction of semivolatiles by non-equilibrium headspace sampling under reduced pressure conditions. Analytica Chimica Acta, 742, 2012, pp. 30–36. doi:10.1016/j.aca.2012.01.019/

12. Adil'bekov E.N., Alimzhanova M.B. (2016) Ekspressnaya metodika skrininga letuchikh organicheskikh zagryaznitelei v vodnykh obraztsakh s ispol'zovaniem metoda TFME/GKh/MS, Izvestiya Natsional'noi akademii nauk Respubliki Kazakhstan. Khimiya i tekhnologiya, no. 420, vol. 6, pp. 65–74.

13. Alimjanova M.B., Qonarbai A.Q., Sergazina M.M. (2018) Zamanaui taldau ädısterı arqyly baldy analitikalyq saraptau: ShOLU // Vestnik Kazakhstansko-Britanskogo tekhnicheskogo universiteta, vol. 15 (2), pp. 139–148.

14. Risticevic S., Lord H., Górecki T. et al. Protocol for solid-phase microextraction method development. Nat Protoc 5, 2010, pp.122–139. https://doi.org/10.1038/nprot.2009.179